Prediction and evaluation of Split open reading frames

The regulation of RNA translation is crucial for maintaining appropriate protein levels within cells. A novel mechanism which controls RNA translation is via the formation of Split open reading frames (Split-ORFs). These occur in transcripts containing at least two open reading frames, each encoding a part of the same full-length protein. These multiple open reading frames arise from alternatively spliced transcript isoforms. We developed the Split-ORF pipeline, a computational tool that predicts Split-ORFs from transcripts’ sequences and identifies regions unique to the predicted Split-ORF products for evaluation with Ribo-seq data.